Sequencing of full-length transcripts in black pepper and small cardamom

Survey of transcriptomes by direct sequencing of fulllength transcripts in the range of 1 to 3 kb was done in black pepper and small cardamom using SMRT Sequencing with PacBio RS II System. Using the PacBio protocol, we obtained 53689 reads of insert (ROIs) with mean read length of 2378 bp in black pepper and 56439 reads of insert with mean read length of 2267 bp in small cardamom. Numbers of polished high-quality isoforms recovered from black pepper and small cardamom transcriptomes were 8,380 and 9,270 respectively. Potential coding regions were also predicted from these sequences.

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